PCR amplification of anEscherichia coligene using mixed primers containing deoxyinosine at ambiguous positions in degenerate amino acid codons

PCR amplification of an Escherichia coli gene using mixed primers containing deoxyinosine at ambiguous positions in degenerate amino acid codons.

Skoog.M.T. 3035 Suzuki.Y. 2935 Walsh,K. 3027 Sokolova,N.I. 3067 Takaku,H2935 Webb.C.E. 3159 Somma.M.P. 2817 Takeda.N. 2841 Whittier,G. 2979 Souhami.R.L. 2929 Tibbetts,C. 3089 Wieben,E.D. 2869 Sparkes,R.S. 2869 Timberlake.W.E. 3105 Wierzchowski,K.L. 2947 Spencer,C. 3152 TittgenJ. 2993 Willcocks.T.C. 3160 Spohr.G. 3081 Tolun.A. 3159 Wilson,W.D. 2979 Stanford,D.R. 2869 Trowsdale.J. 2921 Yasueda,H....

Group-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residue at positions of codon degeneracy.

We have developed a method for differentiating polioviruses from nonpolio enteroviruses using PCR. A pair of panpoliovirus PCR primers were designed to match intervals encoding amino acid sequences within VP1 that are strongly conserved among polioviruses. The initiating primer hybridizes with codons of a 7-amino-acid sequence that has been found only in polioviruses; the second primer matches ...

Group-Specific Identification of Polioviruses by PCR Using Primers Containing Mixed-Base or Deoxyinosine Residues at Positions of Codon Degeneracy

Codon Degeneracy or Deoxyinosine Residues at Positions of by PCR Using Primers Containing Mixed-Base Serotype-Specific Identification of Polioviruses

Designing Of Degenerate Primers-Based Polymerase Chain Reaction (PCR) For Amplification Of WD40 Repeat-Containing Proteins Using Local Allignment Search Method

Degenerate primers-based polymerase chain reaction (PCR) are commonly used for isolation of unidentified gene sequences in related organisms. For designing the degenerate primers, we propose the use of local alignment search method for searching the conserved regions long enough to design an acceptable primer pair. To test this method, a WD40 repeat-containing domain protein from Beauveria bass...